CANADA
Special note for the importation of semen, embryos, oocytes from ruminants :
a)  If intended for in vivo use (artificial insemination, embryo transfer):
The collection of gametes and embryos must be carried out according to the regulations approved for commercial AI and ET centres. See Appendix I for details.
b) If intended for in vitro use only:
The collection of gametes and embryos must be carried out according to the regulations approved for commercial AI and ET centres. Please see Appendix I for details. However, the importer may attach a letter to the permit application with a brief explanation of the sample collection and processing methods, the nature of the work, the end use, and all the conditions in Appendix I that can not be met. The application will be assessed and if the samples pose no zoosanitary risks, an import permit may be issued and the product will be allowed entry under the described conditions.
Special note for the importation of cell lines from ruminants:

Cells lines from countries that are designated as "disease-free", that is, not affected by FMD, BSE, TB, etc., are exempt from import permit restrictions (see chart above). Cell lines from disease-affected countries may be imported under the authority of an import permit. A letter must be attached to the permit application with a brief explanation of the following: description of the cell line, the nature of the work, the method for disposal and the facilities in Canada where the work will be carried out.

Current list of restricted commodities:

 Importation of ruminant or swine semen and embryos (also, organs and tissues) from FMD affected countries is prohibited.
 Importation of ovine and caprine embryos from BSE affected countries is prohibited; bovine embryos may be imported if the country of origin can certify certain conditions required by the CFIA (see their website for conditions required). Semen (bovine, ovine, caprine) are exempt from import requirements specific to BSE.
 Importation of avian products (live birds, hatching eggs and semen) from the states of California and Nevada are currently suspended due to the Newcastle disease outbreak.

CANADA  Appendix I:

CONDITIONS OF IMPORT FOR GERMPLASM FROM RUMINANTS:
(summarized from CFIA regulations - see website for further details)

Zoosanitary export documentation:

 must clearly describe the animal(s) or thing(s) and the country of origin
 must be issued by an inspector of the central veterinary service of the country of origin; or, by a veterinarian designated for such purposes by the central veterinary service of the country of origin and endorsed by an official veterinary inspector of the central veterinary service of the country of origin.
 must include the following details: the registered name, registration number, species and breed of the donor dam and sire, the name and address of the consignor, address of the collection premises, period of residency of the donor dam at the collection premises, name and approval number of semen collection center if artificial insemination was used, date of embryo collection and the number of embryos from each collection date, the total number of embryos in the consignment, total number of straws in consignment, the identification markings or labelling on the straws, the serial number on the shipping tank and the number or markings of the tamper proof seal applied to the shipping container, and the name and address of the consignee.

 

 

 

 

 


Should the disease status of the country of origin change between the time of issuance of this permit and the time of unrestricted entry into Canada, the import shipment may be refused entry into Canada or be subject to additional quarantine and testing or treatment. Importers will be responsible for any additional incurred costs.

 

The donor animal(s) must originate from a herd certified free from:
Embryos/Oocytes: brucellosis 
tuberculosis
vesicular stomatitis virus
anaplasmosis
bluetongue
brucellosis
Semen: vesicular stomatitis virus 
bluetongue
bovine viral diarrhea
brucellosis
campylobacter fetus
enzootic bovine leukosis
pathogenic serotypes of leptospirae
paratuberculosis
tuberculosis

The donor animal(s) of the germplasm presented for importation:
 must have been continually resident in the region, country, or zone of origin either for a minimum of sixty (60) days immediately preceding export or any quarantine period or have been resident since birth or hatching.
 must have been examined and found free from clinical evidence of communicable disease at least thirty (30) days prior to the collection of the germplasm and during every procedure related to the preparation and collection of germplasm.

EMBRYOS/OOCYTES:
 must have been washed, treated and processed in accordance with the protocol detailed in the Manual of the International Embryo Transfer Society (IETS).
 must have been collected and processed by an embryo collection team approved by the central veterinary service of the country of origin.
 must have been conceived by artificial insemination with semen from a donor sire standing at a semen collection centre approved for that purpose by the central veterinary service of the country of origin.
 the entire surface of the zona pellucida of each embryo must have been examined at not less than 50X magnification and found intact and free from adherent material, prior to freezing or shipment of the embryos.
 any biological product of animal origin used in the media and solutions for collection, processing, washing and storage of the embryos/oocytes must be certified free from pathogenic microorganism.
 must have been collected, processed and stored in a hygienic manner that prevented contamination with pathogenic microorganisms.  All material with animal ingredients used in the processing of the germplasm must have been sourced and processed to prevent introduction of pathogenic microorganisms.  All equipment used to collect, handle, wash, freeze and store the germplasm presented for import must have been new, or sterilized prior to use.
 must be stored in a sealed container in ampules, straws or other receptacles indelibly marked in accordance with the recommendations of the International Embryo Transfer Society (IETS) for labeling.

 

 

 

 

 

 

 

NOTE: if the embryos were collected, washed, treated and processed in accordance with the protocol prescribed by the International Zoo Sanitary Code, of the O.I.E., Appendix 4.2.3.1, by an Embryo Transfer Team under the supervision of a team veterinarian officially approved under the E.E.C. guidelines for the export of embryos by the United States Department of Agriculture or certified by the American Embryo Transfer Association, the requirements for the individual testing of the donor dam for tuberculosis, brucellosis, bluetongue and anaplasmosis listed on this permit do NOT have to be fulfilled.

SEMEN:
 must have been collected and processed in a centre approved for such purposes by the central veterinary service of the country of origin.
 must have been extended with a diluent containing one of the combinations of antibiotics listed below. Each ml of extended semen must have a final concentration of antibiotics not less than specified below.
Option 1: 500 IU per ml streptomycin, 500 IU per ml penicillin, 150 ug per ml lincomycin, 300 ug per ml spectinomycin. Immediately after the addition of the extender, the semen must have been held at a temperature of not less than 5 degrees Celsius / 41 degrees Fahrenheit (for semen to be frozen) or 15 degrees Celsius (for fresh semen) for at least 45 minutes.
Option 2: 50 ug per ml tylosin, 250 ug per ml gentomycin, 150 ug per ml lincomycin, 300 ug per ml spectinomycin. The undiluted semen must have been in contact with the antibiotic for not less than three (3) minutes. The semen and the non?glycerol fraction of the diluent must have been held at a temperature of not less than 5 degrees Celsius / 41 degrees Fahrenheit for at least two (2) hours.
Option 3: Any antibiotic combination that can be demonstrated to have an equivalent effect.
 must have been collected, processed and stored in a hygienic manner that prevented contamination with pathogenic microorganisms.  All material with animal ingredients used in the processing of the germplasm must have been sourced and processed to prevent introduction of pathogenic microorganisms.  All equipment used to collect, handle, wash, freeze and store the germplasm presented for import must have been new, or sterilized prior to use.
 the straws or ampules must be sealed at the time of freezing.
 must be in individual receptacles or straws, each marked with the collection date, breed and identity of the donor and the identity of the semen collection center.