DABE Virtual Preconference Workshop

Extracellular Vesicles in Biomedical Embryology
January 18, 2021

Introduction to workshop - Marcia Ferraz and Beatriz Rodriguez Alonso

Extracellular vesicles isolation and characterization
Dr. Kenneth W. Witwer, Associate Professor, Johns Hopkins University School of Medicine
Depts of Molecular and Comparative Pathobiology and Neurology
Executive Chair of Science and Meetings, International Society for Extracellular Vesicles

Part 1: Methods for isolation and quantification
Part 2: MISEV guidelines for EVs experiments

Micro-engineering EVs
Dr. Ke Cheng, Randall B. Terry, Jr. Distinguished Professor in Regenerative Medicine
Dept. of Molecular Biomedical Sciences, North Carolina State University
Dept. of Biomedical Engineering, University of North Carolina - Chapel Hill and North Carolina State University
Division of Molecular Pharmaceutics, University of North Carolina - Chapel Hill

Gene editing using EVs
Dr. Randy Schekman, Department of Molecular and Cell Biology
University of California at Berkeley
Nobel Prize in Physiology or Medicine 2013 for his discoveries of machinery regulating vesicle traffic

Morulas-DABE Science SLAM, 5 minute presentations

Validation of candidate mutation responsible for embryonic lethality in Holstein haplotype 2 carriers, Kelsey Clark (Abstract 96)
High-resolution ribosome profiling reveals translational selectivity in the mammalian blastocyst, Linkai Zhu (Abstract 47)
SIN3A regulates porcine early embryonic development by modulating CCNB1 expression, Kun Zhang (Abstract 64)
Correction of CFTR/G542X mutation using CRISPR/Cas9 genome editing in ovine-bovine interspecies Embryos, Zhiqiang Fan (Abstract 92)
Analysis of miRNA content of oviduct and uterine extracellular vesicles across the bovine estrous cycle, Meriem Hamdi (Abstract 36)
Embryo aggregation and adipose-derived mesenchymal donor cells in bovine SCNT, Virgilia Alberio (Abstract 16)

Science SLAM Awards and closing remarks

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