Monday, January 10

IETS Preconference Symposium

Chairs, Dr. M.B. Wheeler and Dr. B.R. Lindsey

The Life and Travels of the IVF Embryo: From Donor to Recipient
Focus on the Practitioner

Part 1

Ovum Pick-Up (OPU) - Set-up and Equipment

This activity will be a live-streamed or taped segment covering the different ultrasound equipment available along with the probes, needles and tubing set-ups used for OPU. We will have equipment from different manufacturers and several practitioners that use the specific equipment. The equipment and set-up will be demonstrated cow-side at Chessie Creek Farm in South Carolina. The workshop participants will have the opportunity to ask the practitioners questions regarding equipment use and set-up at the workshop room in Savannah, GA. The equipment vendors will have the equipment available.

The emphasis for Part 1 will be with all things on the COW-SIDE, with respect to the aspirator and an assistant performing anything related to donor prep, maintenance of sterile technique and temperature control, the OPU itself, recording relevant info/data; basically everything leading up to the point of handing over the oocyte collection vessel to the searching lab.

Part 2

Recovery and Transport of the Oocyte to the Laboratory

We will have equipment from different manufactures and several practitioners that use the specific equipment. The equipment and set-up will be demonstrated at the Conference Hotel in Savannah GA. The workshop participants will have the opportunity to use and ask the practitioners questions regarding equipment use and set-up at the workshop room in Savannah, GA. The equipment vendors will have the equipment available.

The emphasis for Part 2 will be primarily on the setup in the LAB to prepare the various media (recovery, rinsing, washing, maturation), rinsing the collection tube/filter, searching, grading, packaging, loading incubator, recording info/data and shipping. Also, to emphasize maintenance of sterile technique and temperature control.

Lunch – On your own

Part 3

Decisions for Packaging & Distribution of IVEP embryos to the Practitioner

The different methods to handle IVEP embryos after production will be demonstrated and discussed. The use of field incubators, embryo freezing and various packaging systems will be covered. We will have equipment from different manufactures and several practitioners that use the specific equipment. The equipment and set-up will be demonstrated at the Conference Hotel in Savannah GA. The workshop participants will have the opportunity to use and ask the practitioners questions regarding equipment use and set-up at the workshop room in Savannah, GA. The equipment vendors will have the equipment available.

Part 3 will emphasize the various scenarios that require decisions in the LAB determine WHEN to pull the embryos out to freeze/transfer due to the variety of logistical concerns of physical distance from the lab to recipient, and number of available recipients, Obviously, this brings into account an emphasis on COMMUNICATIONS between the lab personnel, the owner of the embryos, the owner/manager of the recipients, potentially a courier or shipper service AND the practitioner who will transfer the embryos.

Part 4

Disposition of IVEP Embryos in the Field

The different methods to package IVEP embryos after production for shipment to the field practitioner will be demonstrated and discussed. The use of field incubators, transport incubators and various embryo delivery systems will be covered. We will have equipment from different manufactures and several practitioners that use the specific equipment. The equipment and set-up will be demonstrated at the Conference Hotel in Savannah GA. The workshop participants will have the opportunity to use and ask the practitioners questions regarding equipment use and set-up at the workshop room in Savannah, GA. The equipment vendors will have the equipment available.

Part 4 will emphasize the handling of the embryos once received from the lab, all the way through to the transfers. Thus, potentially, unloading embryos out of tubes and into straws. Thus, this part should probably also include discussions of temperature control, organization and coordination of unloading tubes, etc. to ensure optimal throughput, recording info/data, etc. Final Group Discussion

CANDES Preconference Symposium

Chair: Dragos Scarlet

Connecting the Spots: understanding cheetah biology to improve reproduction

Adrienne Crosier, USA

Success rate in a clinical equine in vitro embryo production program

Anthony Claes, Netherlands

Wild theriogenology: understanding reproduction of natural populations in the context of conservation physiology

Karina Acevedo-Whitehouse, Mexico

Recovering a critically endangered frog species using assisted reproductive and genetic technologies

Natalie Calatayud, Australia

Past, present and future of assisted reproduction in rhinoceros - from fertility assessment to parturition

Robert Hermes, Germany

Program

48th Annual Conference of the IETS
January 10-13, 2022

Program Co-Chairs: Hilde Aardema and Flavio Vieira Meirelles

Theme: Adaptation of early life to prepare for healthy future

Tuesday, January 11

Session I: The optimal environment for gametes and the proper environment

Metabolic exchanges between the oocyte and its environment: focus on lipids
Svetlana Uzbekova, France

Physiological parameters related to oocyte nuclear differentiation for the improvement of IVM/IVF outcomes in women and cattle
José Buratini, Brazil

Session II: Maternal conditions affecting future performance in practice

Impact of oocyte donor age and breed on in vitro embryo production in cattle, and relationship of dairy and beef embryo recipients on pregnancy and the subsequent performance of offspring: a review
Pietro Baruselli, Brazil

Female age and parity in horses: how and why does it matter?
Pascale Chavatte-Palmer, France

Wednesday, January 12

Session III: Methods to mimic the in vivo environment in vitro

Role of reproductive fluids and extracellular vesicles in embryo-maternal interaction during early pregnancy in cattle
Dimitrios Rizos, Spain

Sperm interaction with the uterine innate immune system: toll-like receptor 2 (TLR2) is a main sensor in cattle
Akio Miyamoto, Japan

Session IV: Modifications to the epigenome for healthy offspring via the male and female gametes

Nurturing the egg: the essential connection between cumulus cells and the oocyte
Claude Robert, Canada

Sperm-borne sncRNAs: potential biomarkers of semen fertility?
Eli Sellem, France

Concurrent Forum

Practitioners’ Forum – (continuation of the Preconference)

Part 5

How Does the Practitioner Set-Up Donor Cows for IVEP: Working with the Farm Manager and the Cattle Owner

Various System and Protocols will be discussed by a panel of expert practitioners with participation by the audience. A consensus bullet point list of best practices will be developed by the group.

Part 6

How Does the Practitioner Set-Up Recipient Cattle for IVEP: Working with the Farm Manager and the Cattle Owner

Various System and Protocols will be discussed by a panel of expert practitioners with participation by the audience. A consensus bullet point list of best practices will be developed by the group.

Concurrent Forum

DABE

Introduction to the session

Updates on genetically tailored animals as disease models and organ donors for xenotransplantation
Eckhard Wolf, Ludwig-Maximilians-Universität München

Exendin Imaging of islet cells in diabetic pigs – TBD

IThera optoacustic device for non-invasive imaging in pigs and rodents
Alexa Hasenbach, European Institute for Molecular Imaging

Genetic modification of stem cells for infrared fluorescent protein expression applicable for optoacustic detection of transplanted cells
Andras Dinnyés, BioTalentum Ltd and Szent Istvan University

Generation of multifunctional nanoparticles for enhanced imaging properties of transplanted cells
Laura Russo, University of Milano-Bicocca

Closing remarks

GALA PARTY (this will be on Wednesday evening this year)

Thursday, January 13

Session V: How to recognize a vital gamete and embryo

Extracellular vesicles-coupled MicroRNAs as mediators of the effect of physiological and environmental factors on bovine oocytes and embryos
Dawit Tesfaye, USA

Parameters to identify good quality oocytes and embryos
Christine Wrenzycki, Germany

Session VI: Keynote lecture

How the environment affects early embryonic development
Marc-Andre Sirard, Canada